Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Human gut bacteria tailor extracellular vesicle cargo for the breakdown of diet- and host-derived glycans.

doi: 10.1073/pnas.2306314120

Figure Lengend Snippet: Fig. 4. Bt modulates OMV cargo in different nutrient conditions. Coomassie blue staining after SDS-PAGE of Bt OM and OMV (V) fractions obtained after growth in minimal media supplemented with levan, mannan, heparin, mucin, hyaluronan, starch, or glucose. Fractions were normalized by optical density (OD) at 600 nm (Materials and Methods).

Article Snippet: Carbohydrates used to supplement MM include glucose (G7528, Sigma), levan (P- Levan, Neogen corp.), mannan (M7504, Sigma) starch (S2004, Sigma), hyaluronan (FH76335, Biosynth Carbosynth), heparin (YH09354, Biosynth Carbosynth), and mucin (M2378, Sigma).

Techniques: Staining, SDS Page, Starch

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Human gut bacteria tailor extracellular vesicle cargo for the breakdown of diet- and host-derived glycans.

doi: 10.1073/pnas.2306314120

Figure Lengend Snippet: Fig. 6. OMV cargo is tailored for the digestion of specific polysaccharides. Heat maps of protein levels (Z-score) showing most enriched (red) and excluded (blue) proteins found in OMVs from Bt grown in minimal media supplemented with levan (Lev), mannan (Man), heparin (Hep), mucin (Muc), hyaluronan (Hyal), or glucose (Glc) as carbon sources. Five biological replicates were performed per growth condition.

Article Snippet: Carbohydrates used to supplement MM include glucose (G7528, Sigma), levan (P- Levan, Neogen corp.), mannan (M7504, Sigma) starch (S2004, Sigma), hyaluronan (FH76335, Biosynth Carbosynth), heparin (YH09354, Biosynth Carbosynth), and mucin (M2378, Sigma).

Techniques:

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Human gut bacteria tailor extracellular vesicle cargo for the breakdown of diet- and host-derived glycans.

doi: 10.1073/pnas.2306314120

Figure Lengend Snippet: Fig. 8. Tailored OMVs from Bt grown in a specific glycan enhance growth of Bacteroides spp. under the same culture condition. Growth curves of Bo, Pv, and Bf in minimal media with levan, hyaluronan, starch, or mucin. Cultures were not supplemented (blue lines), supplemented with 1 μg/mL of Bt OMVs obtained after growth in the same glycan (red lines), or supplemented with 1 μg/mL of Bt OMVs obtained after growth in a different glycan (green lines).

Article Snippet: Carbohydrates used to supplement MM include glucose (G7528, Sigma), levan (P- Levan, Neogen corp.), mannan (M7504, Sigma) starch (S2004, Sigma), hyaluronan (FH76335, Biosynth Carbosynth), heparin (YH09354, Biosynth Carbosynth), and mucin (M2378, Sigma).

Techniques: Glycoproteomics, Starch

Journal: iScience

Article Title: Effective wound healing on diabetic mice by adhesive antibacterial GNPs-lysine composited hydrogel

doi: 10.1016/j.isci.2024.108860

Figure Lengend Snippet:

Article Snippet: Hyaluronic acid sodium slat-Average MW 1.0-2.0 million Da , BIOSYNTH Carbosynth , CAS No:[9067-32-7].

Techniques: Virus, Recombinant, Membrane, Saline, SYBR Green Assay, CCK-8 Assay, Staining, Derivative Assay, Software, Real-time Polymerase Chain Reaction

Journal: Carbohydrate polymers

Article Title: 3D bioprinted colorectal cancer models based on hyaluronic acid and signalling glycans.

doi: 10.1016/j.carbpol.2022.120395

Figure Lengend Snippet: Scheme 1. Schematic structures of 1A) gelatin functionalized with azide group (Gel–N3), 2A) gelatin functionalized with azide group and 3′-Sialylgalactose (GelN3- 3′-SialGal), 3A) gelatin functionalized with azide group and 6′-Sialylgalactose (GelN3-6′-SialGal), 4A) gelatin functionalized with azide group and 2′-Fucosylgalactose (GelN3-2′-FucGal); B) final hydrogel structure obtained crosslinking glyconjugate-azide-gelatin derivatives and azide-conjugate hyaluronic acid with 4arm- PEG-DBCO.

Article Snippet: 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl) (DMTMM) were purchased from TCI; Alexa Fluor 647 Phalloidin, 4′,6-diamidino-2phenylindole (DAPI) from Thermo Fisher Scientific; CD133/1 (AC133) pure human from Miltenyi Biotec; Human LGR5/GPR49 Antibody (MAB8078) was purchased from Bio-techne brand; Intesticult medium from Stem cell technologies; Buffer solution pH 6.0 (20 ◦C) citric acid / sodium hydroxyde solution with fungicide from Fisher Scientific; Hyaluronic acid (YH05852 average MW 1.0–2.0 million Da) from Carbosynth Ltd.; Human colon cancer cells HT29 from LGC Standards S.r.L.

Techniques: